Antibody to Caspase-2 was used at 1:750 to detect rat caspase-2 in transfected human 292 cell lysates by WB. Asterisk indicates a 48 kDa caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amershams substrate system.