Illustration of the assay principle.
Illustration of the assay principle.
Cathepsin B Inhibitor Screening Assay Kit
79590
Product group Assays
Overview
- SupplierBPS Bioscience
- Product NameCathepsin B Inhibitor Screening Assay Kit
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe Cathepsin B Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin B for screening and profiling applications. The Cathepsin B assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin B (amino acids 18-339), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
- Storage InstructionSee manual
- UNSPSC41116133
References
- Aggarwal, N., and Sloane, B. F. (2014). Cathepsin B: multiple roles in cancer. PROTEOMICS- Clinical Applications 8(5-6), 427-437
- Siklos, M., BenAissa, M., and Thatcher, G. R. (2015). Cysteine proteases as therapeutic targets: does selectivity matter? A systematic review of calpain and cathepsin inhibitors. Acta Pharmaceutica Sinica B, 5(6), 506-519