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Rat TCA4 detected by Western Blot in transfected supernatant
Rat TCA4 detected by Western Blot in transfected supernatant
Rat TCA4 detected by Western Blot in transfected supernatant

CCL21 antibody

GTX45119
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCcl21
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Overview

  • Supplier
    GeneTex
  • Product Name
    CCL21 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID298006
  • Target name
    Ccl21
  • Target description
    C-C motif chemokine ligand 21
  • Target synonyms
    C-C motif chemokine 21; Ccl21b; chemokine (C-C motif) ligand 21; chemokine (C-C motif) ligand 21b (serine); small inducible cytokine A21
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CCL21, Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ccl21 using anti-Ccl21 antibody (A00765-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl21 antigen affinity purified polyclonal antibody (Catalog # A00765-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ccl21 at approximately 15 kDa. The expected band size for Ccl21 is at 15 kDa.
Product group Antibodies
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ApplicationsFlow Cytometry, Western Blot, ELISA
TargetCcl21
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