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CCL26 / Eotaxin 3 Antibody

LS-C104370
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCCL26
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CCL26 / Eotaxin 3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Eotaxin-3 (2 ug/ml), a concentration of 1-5 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human Eotaxin-3 when used with biotinylated antigen affinity purified anti-human Eotaxin-3 (LS-C104390) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml. Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.. ELISA (1 - 2 µg/ml), WB (0.25 - 0.5 µg/ml) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Eotaxin-3 (2 ug/ml), a concentration of 1-5 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human Eotaxin-3 when used with biotinylated antigen affinity purified anti-human Eotaxin-3 (LS-C104390) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml. Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10344
  • Target name
    CCL26
  • Target description
    C-C motif chemokine ligand 26
  • Target synonyms
    CC chemokine IMAC; C-C motif chemokine 26; chemokine (C-C motif) ligand 26; chemokine N1; eotaxin-3; IMAC; macrophage inflammatory protein 4-alpha; MIP-4a; MIP-4alpha; MIP-4-alpha; SCYA26; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; thymic stroma chemokine-1; TSC-1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C,2°C to 8°C,RT
  • UNSPSC
    12352203

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Figure 1. IHC analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066). Eotaxin 3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Eotaxin 3 Antibody (PB9066) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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