CCL3 / MIP-1-Alpha Antibody (Biotin)

LifeSpan BioSciences

CCL3 / MIP-1-Alpha Antibody (Biotin)

23

Rat MIP-1alpha

ELISA: To detect Rat MIP-1alpha by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MIP-1alpha. To detect Rat MIP-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat MIP-1alpha (LS-C104774) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MIP-1alpha. Western Blot: To detect Rat MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MIP-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ELISA (0.25 - 1 µg/ml), WB (0.1 - 0.2 µg/ml) ELISA: To detect Rat MIP-1alpha by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MIP-1alpha. To detect Rat MIP-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat MIP-1alpha (LS-C104774) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MIP-1alpha. Western Blot: To detect Rat MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MIP-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Western Blot, ELISA

Research Use Only

Polyclonal

Biotin

...

CCL3

C-C motif chemokine ligand 3

C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; G0S19-1; LD78ALPHA; macrophage inflammatory protein 1-alpha; MIP1A; MIP-1-alpha; PAT 464.1; SCYA3; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); tonsillar lymphocyte LD78 alpha protein

Rabbit

Rat

-20°C,2°C to 8°C,RT

12352203