CCL8 / MCP2 Antibody

LifeSpan BioSciences

CCL8 / MCP2 Antibody

23

Human MCP-2

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.75mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hMCP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MCP-2 (LS-C104722) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-2. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.75mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hMCP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MCP-2 (LS-C104722) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-2. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA

Research Use Only

Polyclonal

Unconjugated

...

CCL8

C-C motif chemokine ligand 8

C-C motif chemokine 8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; monocyte chemoattractant protein 2; monocyte chemotactic protein 2; SCYA10; SCYA8; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); small-inducible cytokine A8

Rabbit

Human

-20°C,2°C to 8°C,RT

12352203