CCN3 / NOV Antibody

LifeSpan BioSciences

CCN3 / NOV Antibody

23

Human NOV

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml-0.50 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hNOV by sandwich ELISA (using 100 &#956.l/well antibody solution) a concentration of 0.5-2 &#956.g/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NOV (LS-C104549) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV. Western Blot: To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml-0.50 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hNOV by sandwich ELISA (using 100 &#956.l/well antibody solution) a concentration of 0.5-2 &#956.g/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NOV (LS-C104549) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV. Western Blot: To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA

Research Use Only

Polyclonal

Unconjugated

...

CCN3

cellular communication network factor 3

CCN family member 3; IBP-9; IGF-binding protein 9; IGFBP9; IGFBP-9; insulin-like growth factor-binding protein 9; nephro blastoma-overexpressed gene protein homolog; nephroblastoma overexpressed; nephroblastoma-overexpressed gene protein homolog; NOV; NOVh; protein NOV homolog

Rabbit

Human

-20°C,2°C to 8°C,RT

12352203