CD103 antibody

Biorbyt

CD103 antibody

16

PBMC: Add 10 microl of MAB/106 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20° C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.

Flow Cytometry

FACS

Research Use Only

Monoclonal

AX.14

Unconjugated

ITGAE

integrin subunit alpha E

antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; HUMINAE; integrin alpha-E; integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); mucosal lymphocyte 1 antigen

Mouse

IgG1

Integrin alpha-E

Mouse monoclonal antibody to CD103

Human

-20°C,2°C to 8°C

12352203