CD11b antibody [OX-42] (Biotin)

GeneTex

CD11b antibody [OX-42] (Biotin)

9

This antibody has been used in Immunohistochemistry (Frozen sections), Flow Cytometry / FACS. Recomended dilutions - FACS: Use neat - 1/10, or 10microl of the suggested working dilution to label 106 cells in 100microl. IHC-Fr: Use at an assay dependent dilution. Note: samples need to be PLP (periodate-lysine-paraformaldehyde) fixed. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen

Research Use Only

Monoclonal

OX-42

0.1 mg/ml

Biotin

Itgam

integrin subunit alpha M

Cd11b, integrin alpha-M, integrin, alpha M

Mouse

IgG2a

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.GTX53827 recognises CD11b expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.

Rat

-20°C or -80°C,2°C to 8°C

41116161