CD152 [CTLA-4] (human) (rec.) (His) (Prod. No. AG-40B-0228) binds to the receptor CD80 (human): Fc (mouse) (rec.) (Prod. No. CHI-HF-211CD80). Method: CD80 (human): Fc (mouse) (rec.) (Prod. No. CHI-HF-211CD80) is coated on an ELISA plate at 1 microg/ml overnight at 4°C. CD152 [CTLA-4] (human) (rec.) (His) (Prod. No. AG-40B-0228) (red line) or a control protein (His) (green line) is incubated (starting at a concentration of 8,000 ng/ml with a two-fold serial dilution) during one hour at RT and the interaction is then detected using an anti-His-(HRP).
CD152 [CTLA-4] (human) (rec.) (His) (Prod. No. AG-40B-0228) binds to the receptor CD80 (human): Fc (mouse) (rec.) (Prod. No. CHI-HF-211CD80). Method: CD80 (human): Fc (mouse) (rec.) (Prod. No. CHI-HF-211CD80) is coated on an ELISA plate at 1 microg/ml overnight at 4°C. CD152 [CTLA-4] (human) (rec.) (His) (Prod. No. AG-40B-0228) (red line) or a control protein (His) (green line) is incubated (starting at a concentration of 8,000 ng/ml with a two-fold serial dilution) during one hour at RT and the interaction is then detected using an anti-His-(HRP).
CD152 [CTLA-4] (human) (rec.) (His)
AG-40B-0228
Protein IDP16410
Product group Proteins / Signaling Molecules
Overview
- SupplierAdipoGen Life Sciences
- Product NameCD152 [CTLA-4] (human) (rec.) (His)
- Delivery Days Customer10
- CertificationResearch Use Only
- Concentration1 mg/ml
- Estimated Purity>95%
- Protein IDP16410
- Protein NameCytotoxic T-lymphocyte protein 4
- Scientific DescriptionCD152 (Cytotoxic T-lymphocyte-associated protein 4; CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T-cells. Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T-cell homeostasis and self-tolerance. While CD28 promotes T-cell activation and proliferation, CTLA-4 is reported to dampen T-cell responses through a variety of mechanisms. Prior to activation, conventional T-cells (Tconv) express low levels of CTLA-4, predominantly in intracellular compartments. Upon activation, CTLA-4 expression is upregulated and becomes increasingly detectable on the cell surface. In Tregs on the other hand, transmembrane CTLA-4 is constitutively expressed and plays an important role in Treg homeostasis and function. In general, T-cell CTLA-4 is largely constrained to intracellular expression although some surface expression may be detectable owing to the rapid, continuous shuttling of CTLA-4 between intracellular compartments and the plasma membrane. Recently, roles of CTLA-4 have been demonstrated in dendritic cells and tumors, showing that CTLA-4 plays nonredundant and critical roles in thymic development, T-cell priming, peripheral tolerance, and a variety of other critical immunoregulatory functions as an immune checkpoint in Immuno-oncology research. - Recombinant Protein. Human CD152 [CTLA-4] (aa 36-161) is fused at the C-terminus to a His-tag. Source: W2. Lyophilized. Contains PBS. CD152 (Cytotoxic T-lymphocyte-associated protein 4; CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T-cells. Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T-cell homeostasis and self-tolerance. While CD28 promotes T-cell activation and proliferation, CTLA-4 is reported to dampen T-cell responses through a variety of mechanisms. Prior to activation, conventional T-cells (Tconv) express low levels of CTLA-4, predominantly in intracellular compartments. Upon activation, CTLA-4 expression is upregulated and becomes increasingly detectable on the cell surface. In Tregs on the other hand, transmembrane CTLA-4 is constitutively expressed and plays an important role in Treg homeostasis and function. In general, T-cell CTLA-4 is largely constrained to intracellular expression although some surface expression may be detectable owing to the rapid, continuous shuttling of CTLA-4 between intracellular compartments and the plasma membrane. Recently, roles of CTLA-4 have been demonstrated in dendritic cells and tumors, showing that CTLA-4 plays nonredundant and critical roles in thymic development, T-cell priming, peripheral tolerance, and a variety of other critical immunoregulatory functions as an immune checkpoint in Immuno-oncology research.
- Storage Instruction2°C to 8°C,-20°C
- UNSPSC12352202