CD20 Antibody Cocktail

Biorbyt

CD20 Antibody Cocktail

16

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody cocktail to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Flow cytometry: 5-10ul/million cells,Immunofluorescence: 1:50-1:100,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT FACS, IF, IHC-P

Research Use Only

Monoclonal

L26 + IGEL/733

MS4A1

membrane spanning 4-domains A1

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; CVID5; FMC7; LEU-16; leukocyte surface antigen Leu-16; membrane-spanning 4-domains, subfamily A, member 1; S7

Mouse

IgG2a

B-lymphocyte antigen CD20

This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). The antibody recognizes the extracellular domain of the protein. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.

Human

12352203