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ProductsBack/CD200 Antibody (clone OX-90, Low Endotoxin)

CD200 Antibody (clone OX-90, Low Endotoxin)

LS-C188205
LifeSpan BioSciences
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetCD200
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD200 Antibody (clone OX-90, Low Endotoxin)
  • Delivery Days Customer
    14
  • Application Supplier Note
    The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
  • Applications
    Flow Cytometry, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    ELISA, Flo (1:10 - 1:50), IHC-Fr The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-90
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4345
  • Target name
    CD200
  • Target description
    CD200 molecule
  • Target synonyms
    antigen identified by monoclonal antibody MRC OX-2; CD200 antigen; MOX1; MOX2; MRC; OX-2; OX-2 membrane glycoprotein
  • Host
    Rat
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD200/OX2 using anti-CD200/OX2 antibody (A01512-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD200/OX2 antigen affinity purified polyclonal antibody (Catalog # A01512-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD200/OX2 at approximately 45KD. The expected band size for CD200/OX2 is at 31KD.
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