Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7 (TA385702). Jurkat cell extract (35microg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 16E7 (TA385702) at 0.1 microg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa, though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. TA385702 successfully detected the canonical human DC-SIGNR, as well as multiple other isoforms.