CD30 Antibody Cocktail

Biorbyt

CD30 Antibody Cocktail

16

Optimal dilution of the CD30 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT (1) IHC-P

Research Use Only

Monoclonal

Ber-H2 + CD30/412

TNFRSF8

TNF receptor superfamily member 8

CD30; CD30L receptor; cytokine receptor CD30; D1S166E; Ki-1; Ki-1 antigen; lymphocyte activation antigen CD30; tumor necrosis factor receptor superfamily member 8

Mouse

IgG

Tumor necrosis factor receptor superfamily member 8

Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkins disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This mAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).

Human

-20°C,2°C to 8°C

12352203