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IHC-Fr analysis of rat brain tissue using GTX74899 CD31 antibody [TLD-3A12].
IHC-Fr analysis of rat brain tissue using GTX74899 CD31 antibody [TLD-3A12].
IHC-Fr analysis of rat brain tissue using GTX74899 CD31 antibody [TLD-3A12].

CD31 antibody [TLD-3A12]

GTX74899
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Primate, Rat
TargetPecam1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD31 antibody [TLD-3A12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-Fr: 1/10-1/100. FCM: 1/10-1/100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TLD-3A12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID29583
  • Target name
    Pecam1
  • Target description
    platelet and endothelial cell adhesion molecule 1
  • Target synonyms
    CD31, Pecam, platelet endothelial cell adhesion molecule, PECAM-1, platelet/endothelial cell adhesion molecule 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ3SWT0
  • Protein Name
    Platelet endothelial cell adhesion molecule
  • Scientific Description
    cell adhesion molecule, also intracellular signaling molecule; may mediate leukocyte adhesion and migration, angiogenesis, and thrombosis [RGD, Feb 2006]
  • Reactivity
    Human, Mouse, Porcine, Primate, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of Pecam1 using anti-Pecam1 antibody (A01513-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pecam1 antigen affinity purified polyclonal antibody (Catalog # A01513-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pecam1 at approximately 120-130KD. The expected band size for Pecam1 is at 82KD.
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