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CD31 antibody [WM59] (FITC)

GTX75301
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetPECAM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD31 antibody [WM59] (FITC)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: Neat. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    WM59
  • Concentration
    0.1 mg/ml
  • Conjugate
    FITC
  • Gene ID5175
  • Target name
    PECAM1
  • Target description
    platelet and endothelial cell adhesion molecule 1
  • Target synonyms
    CD31; CD31 antigen; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16284
  • Protein Name
    Platelet endothelial cell adhesion molecule
  • Scientific Description
    The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • TREM2 activation alleviates neural damage via Akt/CREB/BDNF signalling after traumatic brain injury in mice.
    Read more
  • S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage. Fan X et al., 2022, Front Pharmacol
    Read more
  • Reducing the Damage of Ox-LDL/LOX-1 Pathway to Vascular Endothelial Barrier Can Inhibit Atherosclerosis. Guo X et al., 2022, Oxid Med Cell Longev
    Read more
  • Exosomal microRNA-1246 from human umbilical cord mesenchymal stem cells potentiates myocardial angiogenesis in chronic heart failure. Wang Z et al., 2021 Nov, Cell Biol Int
    Read more
  • Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head. Xu H et al., 2021 May, Stem Cells Transl Med
    Read more
  • Bone marrow donor selection and characterization of MSCs is critical for pre-clinical and clinical cell dose production. Trivedi A et al., 2019 Apr 17, J Transl Med
    Read more
  • Runx3 inhibits endothelial progenitor cell differentiation and function via suppression of HIF-1alpha activity. Choo SY et al., 2019 Apr, Int J Oncol
    Read more
  • Chitosan/silk fibroin modified nanofibrous patches with mesenchymal stem cells prevent heart remodeling post-myocardial infarction in rats. Chen J et al., 2018 Oct 15, Acta Biomater
    Read more
  • EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma. Lee HY et al., 2018 Mar 2, Oncotarget
    Read more
  • Recombinant Thrombomodulin Exerts Anti-autophagic Action in Endothelial Cells and Provides Anti-atherosclerosis Effect in Apolipoprotein E Deficient Mice. Chen PS et al., 2017 Jun 12, Sci Rep
    Read more

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Figure 1. Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PB9094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31/PECAM1 antigen affinity purified polyclonal antibody (Catalog # PB9094) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31/PECAM1 at approximately 90 kDa, 130 kDa. The expected band size for CD31/PECAM1 is at 82 kDa.
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