Flow cytometry using the Anti-CD32 antibody IV.3 (TA386512). Paraformaldehyde fixed U937 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (TA385792; isotype control, black line) or the rabbit IgG version of IV.3 (TA386512, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Flow cytometry using the Anti-CD32 antibody IV.3 (TA386512). Paraformaldehyde fixed U937 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (TA385792; isotype control, black line) or the rabbit IgG version of IV.3 (TA386512, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
CD32A (FCGR2A) Rabbit Monoclonal Antibody [Clone ID: IV.3]
TA386512
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman
TargetFCGR2A
Overview
- SupplierOriGene
- Product NameCD32A (FCGR2A) Rabbit Monoclonal Antibody [Clone ID: IV.3]
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDIV.3
- Gene ID2212
- Target nameFCGR2A
- Target descriptionFc fragment of IgG receptor IIa
- Target synonymsCD32; CD32A; CDw32; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc gamma receptor RIIa3; FCG2; fc-gamma-RIIa; FcGR; FCGR2; FCGR2A1; fcRII-a; IGFR2; igG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a
- HostRabbit
- IsotypeIgG
- Scientific DescriptionRecombinant Anti-CD32 (Clone IV.3)
- ReactivityHuman
- UNSPSC12352203