CD33 antibody

Biorbyt

CD33 antibody

16

1. Take 100 microl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Flow Cytometry, ImmunoFluorescence

FC, IF

Research Use Only

Monoclonal

6C5

Unconjugated

CD33

CD33 molecule

CD33 antigen (gp67); CD33 molecule transcript; gp67; myeloid cell surface antigen CD33; p67; sialic acid-binding Ig-like lectin 3; SIGLEC3; SIGLEC-3

Mouse

Myeloid cell surface antigen CD33

Mouse monoclonal antibody to CD33

Human

-20°C,2°C to 8°C

12352203