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CD34 Antibody (aa318-328)

LS-C155057
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCD34
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD34 Antibody (aa318-328)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments in Human Umbilical Cord and peripheral lymphocytes lysates gave no specific signal but low background (at antibody concentration up to 1 ug/ml).. Peptide-ELISA (1:128000), WB (1 - 3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments in Human Umbilical Cord and peripheral lymphocytes lysates gave no specific signal but low background (at antibody concentration up to 1 ug/ml).
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID947
  • Target name
    CD34
  • Target description
    CD34 molecule
  • Target synonyms
    CD34 antigen; hematopoietic progenitor cell antigen CD34
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
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