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CD40L (human) ELISA Kit

Research Use Only
AG-45B-0018
AdipoGen Life Sciences
ReactivityHuman
Product group Assays
Price on request
Packing Size
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    CD40L (human) ELISA Kit
  • Delivery Days Customer
    10
  • Applications
    ELISA
  • Assay Detection Range
    0.03125 to 2ng/ml
  • Assay Sensitivity
    20pg/ml
  • Assay Specificity
    Detects soluble human CD40L in serum, plasma and cell culture supernatant.
  • Certification
    Research Use Only
  • Scientific Description
    CD40 (also called, Tumor necrosis factor receptor superfamily member 5 or TNFRSF5), a transmembrane type I glycoprotein, belongs to the tumor necrosis factor (TNF) gene superfamily and behaves as a co-stimulatory molecule found on B cells, monocytes, antigen presenting cells, endothelial, smooth muscle cells, fibroblasts and platelets. Its ligand, CD40L (also called CD154), a type II transmembrane protein, is a 261 amino acid (aa) glycoprotein that forms homotrimers. CD40L is found on activated T cells, B cells, platelets, endothelial, epithelial and smooth muscle cells. Proteolytic cleavage can produce a soluble form of CD40L (sCD40L). Platelets are the main source of sCD40L. CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production and isotype switching, formation of B memory cells and germinal centers. It also prevents apoptosis of germinal center B cells. Defective expression of CD40L in humans leads to an inability to produce isotypes other than IgM (hyper IgM syndrome) and to an absence of germinal centers. In dendritic cells, CD40 ligation induces more effective antigen, enhances T-cell stimulatory capacity and induces production of several inflammatory cytokines and chemokines. CD40-CD40L interactions are crucially involved in development of autoimmune disease and have also impact on growth regulation of certain carcinomas. Elevated levels of sCD40L have been observed in sera from patients with systemic lupus erythematosus (SLE), chronic lymphocytic leukemia (CLL) and unstable angina. - ELISA Assay. Detects soluble human CD40L in serum, plasma and cell culture supernatant. Colorimetric sandwich assay. Sample types: Cell Culture Supernatant, Plasma, Serum. Range: 0.015625 to 1ng/ml. Sensitivity: 10pg/ml. CD40 (also called, Tumor necrosis factor receptor superfamily member 5 or TNFRSF5), a transmembrane type I glycoprotein, belongs to the tumor necrosis factor (TNF) gene superfamily and behaves as a co-stimulatory molecule found on B cells, monocytes, antigen presenting cells, endothelial, smooth muscle cells, fibroblasts and platelets. Its ligand, CD40L (also called CD154), a type II transmembrane protein, is a 261 amino acid (aa) glycoprotein that forms homotrimers. CD40L is found on activated T cells, B cells, platelets, endothelial, epithelial and smooth muscle cells. Proteolytic cleavage can produce a soluble form of CD40L (sCD40L). Platelets are the main source of sCD40L. CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production and isotype switching, formation of B memory cells and germinal centers. It also prevents apoptosis of germinal center B cells. Defective expression of CD40L in humans leads to an inability to produce isotypes other than IgM (hyper IgM syndrome) and to an absence of germinal centers. In dendritic cells, CD40 ligation induces more effective antigen, enhances T-cell stimulatory capacity and induces production of several inflammatory cytokines and chemokines. CD40-CD40L interactions are crucially involved in development of autoimmune disease and have also impact on growth regulation of certain carcinomas. Elevated levels of sCD40L have been observed in sera from patients with systemic lupus erythematosus (SLE), chronic lymphocytic leukemia (CLL) and unstable angina.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116133