Bio-Connect
CD45RO(UCHL-1), CF405S conjugate, 0.1mg/mL [26628-22-8]

CD45RO(UCHL-1), CF405S conjugate, 0.1mg/mL [26628-22-8]

BNC040003
Biotium
ApplicationsFunctional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPTPRC
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Biotium
  • Product Name
    CD45RO(UCHL-1), CF405S conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    UCHL-1
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID5788
  • Target name
    PTPRC
  • Target description
    protein tyrosine phosphatase receptor type C
  • Target synonyms
    B220; CD45; CD45 antigen; CD45R; GP180; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP08575
  • Protein Name
    Receptor-type tyrosine-protein phosphatase C
  • Scientific Description
    Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
ApplicationsELISA
TargetPTPRC
  • SizePrice
Western blot analysis of Hela, diluted at 1:2000.
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA
TargetPTPRC
  • SizePrice
Product group Antibodies
Nordic-MUbio
ApplicationsFlow Cytometry
TargetPTPRC
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
MedChem Express
TargetPTPRC
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
TargetPTPRC
  • SizePrice
Figure 1. Western blot analysis of CD45/PTPRC using anti-CD45/PTPRC antibody (A00555-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD45/PTPRC antigen affinity purified polyclonal antibody (Catalog # A00555-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45/PTPRC at approximately 220 kDa. The expected band size for CD45/PTPRC is at 220 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetPTPRC
  • SizePrice
Product group Antibodies
Assay Genie
ApplicationsFlow Cytometry
TargetPTPRC
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPTPRC
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • SizePrice