Bio-Connect
FACS analysis of mouse C57Bl/6 splenocytes using GTX01465-08 CD5 antibody [53-7.3] (PE). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 microg (5 microl)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01465-08 CD5 antibody [53-7.3] (PE). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 microg (5 microl)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01465-08 CD5 antibody [53-7.3] (PE). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 microg (5 microl)

CD5 antibody [53-7.3] (PE)

GTX01465-08
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd5
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    CD5 antibody [53-7.3] (PE)
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    53-7.3
  • Concentration
    0.2 mg/ml
  • Conjugate
    RPE
  • Gene ID12507
  • Target name
    Cd5
  • Target description
    CD5 antigen
  • Target synonyms
    Ly; Ly-; Ly-1; Ly-12; Ly-A; lymphocyte antigen 1; Lyt-; Lyt-1; T-cell surface glycoprotein CD5
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP13379
  • Protein Name
    T-cell surface glycoprotein CD5
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • CD5 expression is developmentally regulated by T cell receptor (TCR) signals and TCR avidity. Azzam HS et al., 1998 Dec 21, J Exp Med
    Read more
  • A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5. Bikah G et al., 1998 Aug, Int Immunol
    Read more
  • CD5-mediated negative regulation of antigen receptor-induced growth signals in B-1 B cells. Bikah G et al., 1996 Dec 13, Science
    Read more
  • A role for CD5 in TCR-mediated signal transduction and thymocyte selection. Tarakhovsky A et al., 1995 Jul 28, Science
    Read more
  • The Ly-1 B cell subpopulation in normal immunodefective, and autoimmune mice. Hayakawa K et al., 1983 Jan 1, J Exp Med
    Read more
  • T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. Ledbetter JA et al., 1980 Aug 1, J Exp Med
    Read more

Related products

FACS analysis of mouse C57Bl/6 splenocytes using GTX01465-06 CD5 antibody [53-7.3] (FITC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.5 microg (5 microl)
Product group Antibodies
References
GeneTex
ApplicationsFlow Cytometry
TargetCd5
  • SizePrice
FACS analysis of mouse C57Bl/6 splenocytes using GTX01465-07 CD5 antibody [53-7.3] (APC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 microg (5 microl)
Product group Antibodies
References
GeneTex
ApplicationsFlow Cytometry
TargetCd5
  • SizePrice
FACS analysis of mouse C57Bl/6 splenocytes using GTX01465-15 CD5 antibody [53-7.3] (APC-Cy7). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 microg (5 microl)
Product group Antibodies
References
GeneTex
ApplicationsFlow Cytometry
TargetCd5
  • SizePrice
Product group Antibodies
Assay Genie
ApplicationsFlow Cytometry
TargetCd5
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoHistoChemistry
TargetCd5
  • SizePrice
Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (A00480-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody (Catalog # A00480-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD5 at approximately 70 kDa. The expected band size for CD5 is at 54 kDa.
Product group Antibodies
Boster Bio
Anti-CD5 Antibody Picoband(r)A00480-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCd5
  • SizePrice