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ProductsBack/CD5 Antibody (clone OTI2D8)

CD5 Antibody (clone OTI2D8)

LS-C336556
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCD5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD5 Antibody (clone OTI2D8)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2D8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID921
  • Target name
    CD5
  • Target description
    CD5 molecule
  • Target synonyms
    CD5 antigen (p56-62); epididymis secretory sperm binding protein; LEU1; lymphocyte antigen T1/Leu-1; T1; T-cell surface glycoprotein CD5
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (A00480-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody (Catalog # A00480-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD5 at approximately 67 kDa. The expected band size for CD5 is at 54 kDa.
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