CD68 Antibody Cocktail

Biorbyt

CD68 Antibody Cocktail

16

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD68 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT IHC-P

Research Use Only

Monoclonal

KP1 + C68/684

Unconjugated

CD68

CD68 molecule

CD68 antigen; GP110; LAMP4; macrophage antigen CD68; macrosialin; SCARD1; scavenger receptor class D, member 1

Mouse

IgG1

This antibody cocktail recognizes a glycoprotein of 110kDa, which is identified as CD68. antibody to CD68 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. CD68 antibody reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. CD68 shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Feline, Human, Mouse, Rat

-20°C,2°C to 8°C

12352203