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ProductsBack/CD69 Antibody (FITC)

CD69 Antibody (FITC)

LS-C123096
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetCD69
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD69 Antibody (FITC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry (frozen). Flow Cytometry: Neat; 20 ul labels 10^6 cells in 100ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. Flo, IF, IHC, IHC-Fr Suitable for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry (frozen). Flow Cytometry: Neat; 20 ul labels 10^6 cells in 100ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID969
  • Target name
    CD69
  • Target description
    CD69 molecule
  • Target synonyms
    activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CLEC2C; C-type lectin domain family 2, member C; EA1; early activation antigen CD69; early lymphocyte activation antigen; early T-cell activation antigen p60; GP32/28; leukocyte surface antigen Leu-23; MLR-3
  • Host
    Mouse
  • Isotype
    IgG1
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD69 using anti-CD69 antibody (A00529-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: mouse SP2/0 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD69 antigen affinity purified polyclonal antibody (Catalog # A00529-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD69 at approximately 22KD. The expected band size for CD69 is at 22KD.
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