![CD97 antibody [HL1924] detects CD97 protein at cell membrane and cytoplasmic vesicles by immunofluorescent analysis. Sample: U937 cells were fixed in ice-cold MeOH for 5 min. Green: CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). CD97 antibody [HL1924] detects CD97 protein at cell membrane and cytoplasmic vesicles by immunofluorescent analysis. Sample: U937 cells were fixed in ice-cold MeOH for 5 min. Green: CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637673/GTX637673_T-44837_20230714_ICC_IF_23072519_958.webp)
CD97 antibody [HL1924] detects CD97 protein at cell membrane and cytoplasmic vesicles by immunofluorescent analysis. Sample: U937 cells were fixed in ice-cold MeOH for 5 min. Green: CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
CD97 antibody [HL1924]
GTX637673
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityCanine, Human
TargetADGRE5
Overview
- SupplierGeneTex
- Product NameCD97 antibody [HL1924]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1924
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID976
- Target nameADGRE5
- Target descriptionadhesion G protein-coupled receptor E5
- Target synonymsCD97, TM7LN1, adhesion G protein-coupled receptor E5, CD97 molecule, leukocyte antigen CD97, seven transmembrane helix receptor, seven-span transmembrane protein, seven-transmembrane, heterodimeric receptor associated with inflammation
- HostRabbit
- IsotypeIgG
- Protein IDP48960
- Protein NameAdhesion G protein-coupled receptor E5
- Scientific DescriptionThis gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
- ReactivityCanine, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637673/GTX637673_45166_20230915_WB_B_23091901_981.webp)
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637673/GTX637673_45166_20230922_WB_D_23100118_388.webp)
![Boiled and unboiled wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Boiled and unboiled wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637673/GTX637673_T-44837_20231006_WB_KO_watermark_23102401_836.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637673/GTX637673_45166_20240112_WB_TPM_watermark_24011618_239.webp)
![CD97 antibody [HL1924] detects CD97 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in MeOH. Green: CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). CD97 antibody [HL1924] detects CD97 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in MeOH. Green: CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637673/GTX637673_45166_20231229_ICC_IF_B_24011618_433.webp)
![Unboiled non-transfected (–) and transfected (+) Expi293 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Unboiled non-transfected (–) and transfected (+) Expi293 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637673/GTX637673_45166_20250515_WB_multiple_B_25052202_902.webp)

![Boiled and unboiled U937 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637674/GTX637674_T-44837_20221125_WB_Fraction_22112723_304.webp)





