CD99 Antibody Cocktail

Biorbyt

CD99 Antibody Cocktail

16

Optimal dilution of the CD99 antibody cocktail should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Flow cytometry: 5-10ul/million cells,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT FACS, IF, IHC-P

Research Use Only

Monoclonal

12E7 + MIC2/877

CD99

CD99 molecule (Xg blood group)

antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; HBA71; MIC2; MIC2 (monoclonal antibody 12E7); MIC2X; MIC2Y; MSK5X; surface antigen MIC2; T-cell surface glycoprotein E2

Mouse

IgG1

CD99 antigen

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors.

Human

-20°C,2°C to 8°C

12352203