Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC37 (phospho Ser13) antibody [GT1338] (GTX03250) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![WB analysis of NIH-3T3 cells were treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03250 CDC37 (phospho Ser13) antibody [GT1338]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03250/GTX03250_95_WB_w_23053123_149.webp "WB analysis of NIH-3T3 cells were treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03250 CDC37 (phospho Ser13) antibody [GT1338]. Dilution : 1:1000 Loading : 25microg per lane")
![WB analysis of C6 cells were treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03250 CDC37 (phospho Ser13) antibody [GT1338]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03250/GTX03250_96_WB_w_23053123_298.webp "WB analysis of C6 cells were treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03250 CDC37 (phospho Ser13) antibody [GT1338]. Dilution : 1:1000 Loading : 25microg per lane")
Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC37 (phospho Ser13) antibody [GT1338] (GTX03250) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CDC37 (phospho Ser13) antibody [GT1338]
GTX03250
Overview
- SupplierGeneTex
- Product NameCDC37 (phospho Ser13) antibody [GT1338] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1338
- Concentration0.09 mg/ml
- ConjugateUnconjugated
- Gene ID11140
- Target nameCDC37
- Target descriptioncell division cycle 37, HSP90 cochaperone
- Target synonymsCDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog; cell division cycle 37 homolog; hsp90 chaperone protein kinase-targeting subunit; hsp90 co-chaperone Cdc37; P50CDC37
- HostRabbit
- IsotypeIgG
- Protein IDQ16543
- Protein NameHsp90 co-chaperone Cdc37
- Scientific DescriptionThe protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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