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Western Blot analysis of 293T cell using CDCA8 Polyclonal Antibody at dilution of 1:900
Western Blot analysis of 293T cell using CDCA8 Polyclonal Antibody at dilution of 1:900
Western Blot analysis of 293T cell using CDCA8 Polyclonal Antibody at dilution of 1:900

CDCA8 Polyclonal Antibody

E-AB-10868
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CDCA8 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDCA8 using anti-CDCA8 antibody (A06612-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCA8 antigen affinity purified polyclonal antibody (Catalog # A06612-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCA8 at approximately 35 kDa. The expected band size for CDCA8 is at 31 kDa.
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