Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CDKN1A Polyclonal Antibody(bs-0741R-CY7),Cy7 Conjugated.at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
CDKN1A Polyclonal Antibody, Cy7 Conjugated
BS-0741R-CY7
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Rat, Sheep
TargetCDKN1A
Overview
- SupplierBioss Antibodies
- Product NameCDKN1A Polyclonal Antibody, Cy7 Conjugated
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateCy7
- Gene ID1026
- Target nameCDKN1A
- Target descriptioncyclin dependent kinase inhibitor 1A
- Target synonymsCAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CIP1; cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; P21; p21CIP1; SDI1; WAF1; wild-type p53-activated fragment 1
- HostRabbit
- IsotypeIgG
- Protein IDP38936
- Protein NameCyclin-dependent kinase inhibitor 1
- ReactivityBovine, Canine, Chicken, Human, Mouse, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203