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CEA Antibody Cocktail

Research Use Only
ORB248301
Biorbyt
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCEACAM5
€ 452,00
20 ug
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Overview

  • Supplier
    Biorbyt
  • Product Name
    CEA Antibody Cocktail
  • Delivery Days Customer
    16
  • Application Supplier Note
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1) (2) IHC-P
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    COL-1 + CEA31 + C66/261
  • Conjugate
    Unconjugated
  • Gene ID1048
  • Target name
    CEACAM5
  • Target description
    CEA cell adhesion molecule 5
  • Target synonyms
    carcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; meconium antigen 100
  • Host
    Mouse
  • Isotype
    IgG1, IgG2a
  • Protein IDP06731
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 5
  • Scientific Description
    This antibody cocktail recognizes proteins of 80-200kDa, identified as different members of the CEA (Carcinoembryonic Antigen) family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. The antibody cocktail shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA antibody positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203