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CENPA Antibody (YA1653)

HY-P81908
MedChem Express
TargetCENPA
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CENPA Antibody (YA1653)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1058
  • Target name
    CENPA
  • Target description
    centromere protein A
  • Target synonyms
    CenH3; CENP-A; centromere autoantigen A; centromere protein A, 17kDa; centromere-specific histone; histone H3-like centromeric protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CENPA Antibody (YA1653) is a non-conjugated IgG antibody, targeting CENPA, with a predicted molecular weight of 16 kDa (observed band size: 16 kDa). CENPA Antibody (YA1653) can be used for WB experiment in human background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CENPA using anti-CENPA antibody (PB9538). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: Rat Pancreas Tissue Lysate at 50ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CENPA antigen affinity purified polyclonal antibody (Catalog # PB9538) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CENPA at approximately 16 kDa. The expected band size for CENPA is at 16 kDa.
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