Competitive ELISA showing the specificity of GTX04680 cGMP antibody [RM467]. The 96-well plate was coated with 1 microg/mL of Goat anti-rabbit IgG (50 microl/well). 0.05 microg/ml of GTX04680 cGMP antibody [RM467] (50 microl/well) was added and incubated. After wash and block, cGMP and other cyclic nucleotides or nucleoside phosphates (25 microl/well) were added along with 25 microl/well of 1:5000 diluted HRP conjugated cGMP.
![Competitive ELISA detecting cGMP in HeLa cells. The 96-well plate was coated with 1 microg/mL of Goat anti-rabbit IgG (50 microl/well). 0.05 microg/ml of GTX04680 cGMP antibody [RM467] (50 microl/well) was added and incubated. After wash and block, HeLa cell lysate (~10?cell/mL) or control (lysate buffer only) (25 microl/well) were added along with 25 microl/well of 1:5000 diluted HRP conjugated cGMP.](https://www.genetex.com/upload/website/prouct_img/normal/GTX04680/GTX04680_20231221_ELISA_2_23122021_280.webp "Competitive ELISA detecting cGMP in HeLa cells. The 96-well plate was coated with 1 microg/mL of Goat anti-rabbit IgG (50 microl/well). 0.05 microg/ml of GTX04680 cGMP antibody [RM467] (50 microl/well) was added and incubated. After wash and block, HeLa cell lysate (~10?cell/mL) or control (lysate buffer only) (25 microl/well) were added along with 25 microl/well of 1:5000 diluted HRP conjugated cGMP.")
![Competitive ELISA of cGMP using GTX04680 cGMP antibody [RM467]. The 96-well plate was coated with 1 microg/mL of Goat anti-rabbit IgG (50 microl/well). 0.05 microg/ml of GTX04680 cGMP antibody [RM467] (50 microl/well) was added and incubated. After wash and block, different concentrations of cGMP (25 microl/well) were added along with 25 microl/well of 1:5000 diluted HRP conjugated cGMP.](https://www.genetex.com/upload/website/prouct_img/normal/GTX04680/GTX04680_20231221_ELISA_23122021_355.webp "Competitive ELISA of cGMP using GTX04680 cGMP antibody [RM467]. The 96-well plate was coated with 1 microg/mL of Goat anti-rabbit IgG (50 microl/well). 0.05 microg/ml of GTX04680 cGMP antibody [RM467] (50 microl/well) was added and incubated. After wash and block, different concentrations of cGMP (25 microl/well) were added along with 25 microl/well of 1:5000 diluted HRP conjugated cGMP.")
Competitive ELISA showing the specificity of GTX04680 cGMP antibody [RM467]. The 96-well plate was coated with 1 microg/mL of Goat anti-rabbit IgG (50 microl/well). 0.05 microg/ml of GTX04680 cGMP antibody [RM467] (50 microl/well) was added and incubated. After wash and block, cGMP and other cyclic nucleotides or nucleoside phosphates (25 microl/well) were added along with 25 microl/well of 1:5000 diluted HRP conjugated cGMP.
cGMP antibody [RM467]
GTX04680
Overview
- SupplierGeneTex
- Product NamecGMP antibody [RM467]
- Delivery Days Customer9
- Antibody SpecificityRM467 reacts to cGMP (Cyclic Guanosine Monophosphate). No cross reactivity with other cyclic nucleotide or nucleoside phosphate.
- Application Supplier NoteELISA: 0.05-0.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM467
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203