Azide-PEG4-aminoallyl-dUTP

Biorbyt

Azide-PEG4-aminoallyl-dUTP

16

Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data; 3-End Labeling with Terminal deoxynucleotidyl Transferase (TdT). Spectroscopic Propertie: lambdamax 289 nm, epsilon 7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Research Use Only

10-11 mM

Azide-PEG 4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing Azide-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice: to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasks; to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for sub-sequent microscopic imaging; to crosslink the DNA to Azide-or DBCO-functionalized biomolecules e.g.proteins.

-20°C

12352200