INDIGlo LDR performance from both Homogenous and Non-Homogenous assays
|
INDIGlo LDR performance from both Homogenous and Non-Homogenous assays
|
Dose-response analyses of Human AhR (INDIGO #IB06001, 96-well assay) was performed using the reference agonist MeBIO. AhR reporter cells were plated across two white assay plates, MeBIO treatment media were added, and the assay plates incubated for 24 hr. Plates were then processed with luciferase detection reagents in either a.) a homogenous or b.) a non-homogenous assay format. Resulting assay metrics are also shown from using another extended-glo detection reagent (Vendor X).
|
INDIGlo LDR potency is stable following repeated freeze and thaw cycles
|
INDIGlo LDR potency is stable following repeated freeze and thaw cycles
|
INDIGlo LDR was frozen at -20°C and thawed either 1, 2 or 3 times, then used to quantify luciferase activity from HEK-293:luc cells cultured in a white 96-well assay plate. No loss of reagent potency is observed between these conditions.
|