INDIGlo Luciferase Detectio... | LDR-10 | INDIGO Biosciences

INDIGlo LDR performance from both Homogenous and Non-Homogenous assays

Dose-response analyses of Human AhR...

INDIGlo LDR potency is stable following repeated freeze and thaw cycles

INDIGlo LDR was frozen at -20°C and...

Product specifications for - INDIGlo Luciferase Detection Reagent Kit, 10 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer

Overview:
Product group:	Chemicals
Category:	Buffers

Properties:
UNSPSC:	12352200
Storage instructions:	-20C

Scientific information:
Scientific info:	INDIGlo Luciferase Detection Reagent (LDR) is formulated to deliver steady, long-lived luminescence signal from mammalian reporter cells expressing firefly luciferase. For convenience, the reagent is provided ‘ready to use’, without the requirement to first re-constitute powder-form components. INDIGlo LDR works equally well in quantifying expressed luciferase activity from 96- or 384-well format reporter assays. The resulting light emission is stable over a 2 hr period (Figure 1), thereby making INDIGlo LDR an ideal, economical detection reagent for both low- and high-throughput batch processing of assay plates. INDIGlo LDR is formulated as a 2x-concentrated reagent, thereby allowing greater versatility in its use. The 2x-concentrated reagent will be used when using 384-well or 1536-well assay plates that are processed using a homogenous assay strategy. Alternatively, when processing 96-well assay plates either a homogenous or non-homogenous assay strategy may be used. For convenience, this kit provides an optimized Dilution Buffer for use in diluting INDIGlo LDR when performing non-homogenous assays. Regardless of one’s assay setup preference INDIGlo LDR delivers rapid lysis of the reporter cells, robust luminescence signal, and sensitive quantification of expressed luciferase activities.

Scientific information:

Scientific info:

INDIGlo Luciferase Detection Reagent (LDR) is formulated to deliver steady, long-lived luminescence signal from mammalian reporter cells expressing firefly luciferase. For convenience, the reagent is provided ‘ready to use’, without the requirement to first re-constitute powder-form components.

INDIGlo LDR works equally well in quantifying expressed luciferase activity from 96- or 384-well format reporter assays. The resulting light emission is stable over a 2 hr period (Figure 1), thereby making INDIGlo LDR an ideal, economical detection reagent for both low- and high-throughput batch processing of assay plates.

INDIGlo LDR is formulated as a 2x-concentrated reagent, thereby allowing greater versatility in its use. The 2x-concentrated reagent will be used when using 384-well or 1536-well assay plates that are processed using a homogenous assay strategy.

Alternatively, when processing 96-well assay plates either a homogenous or non-homogenous assay strategy may be used. For convenience, this kit provides an optimized Dilution Buffer for use in diluting INDIGlo LDR when performing non-homogenous assays.

Regardless of one’s assay setup preference INDIGlo LDR delivers rapid lysis of the reporter cells, robust luminescence signal, and sensitive quantification of expressed luciferase activities.

Additional information:
Synonyms:	LDR-10; INDIGO Biosciences

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INDIGlo Luciferase Detection Reagent Kit, 10 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer

Product specifications for - INDIGlo Luciferase Detection Reagent Kit, 10 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer

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Find the product you need

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INDIGlo Luciferase Detection Reagent Kit, 10 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer

Product specifications for - INDIGlo Luciferase Detection Reagent Kit, 10 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer