LPS from E. coli O8:K27 (S-form) TLRpure Sterile Solution

Catalog number: IAX-100-006-C500
Brand: Innaxon
Packing: 500 ug
Other sizes: Other sizes available
Price: € 97.00
Expected delivery time: 7 days
Quantity:

Product specifications for - LPS from E. coli O8:K27 (S-form) TLRpure Sterile Solution

Overview: 
Product group: Chemicals
Category: Other
Properties: 
Purity: >99.9%. No detectable DNA, RNA and protein traces.
Datasheet: Datasheet
  Research Use Only
UNSPSC: 12352200
Concentration: 1 mg/ml
Form supplied: Liquid. Colourless clear aqueous solution.
Storage instructions: 2-¦C to 8-¦C
Scientific information: 
Scientific info: Activation of cells by LPS is mediated by the Toll-like receptor 4 (TLR4). For optimal interaction with LPS, TLR4 requires association with myeloid differentiation protein 2 (MD-2). According to current consensus activation of TLR4 is preceded by the transfer of LPS to membrane-bound (m) or soluble (s) CD14 by LPS-binding protein (LBP). Re-form LPS and lipid A, but not S-form LPS, are capable of inducing TNF-alpha responses also in the absence of CD14. LPS, synthesized by most wild-type (WT) Gram-negative bacteria (S-form LPS), consists of three regions, the O-polysaccharide chain, which is made up of repeating oligosaccharide units, the core oligosaccharide and the lipid A, which harbors the endotoxic activity of the entire molecule. R-form LPS synthesized by the so-called rough (R) mutants of Gram-negative bacteria lacks the O-specific chain. Furthermore, the core-oligosaccharide may be present in different degrees of completion, depending on the class (Ra to Re) to which the mutant belongs. LPS are amphipathic molecules whose hydrophobicity decreases with increasing length of the sugar part. Based upon these differences, S- and R-form LPS show marked differences in the kinetics of their blood clearance and cellular uptake as well as in the ability to induce oxidative burst in human granulocytes and to activate the host complement system. - Chemical. Isolated and purified from E. coli O8:K27. Activation of cells by LPS is mediated by the Toll-like receptor 4 (TLR4). For optimal interaction with LPS, TLR4 requires association with myeloid differentiation protein 2 (MD-2). According to current consensus activation of TLR4 is preceded by the transfer of LPS to membrane-bound (m) or soluble (s) CD14 by LPS-binding protein (LBP). Re-form LPS and lipid A, but not S-form LPS, are capable of inducing TNF-alpha responses also in the absence of CD14. LPS, synthesized by most wild-type (WT) Gram-negative bacteria (S-form LPS), consists of three regions, the O-polysaccharide chain, which is made up of repeating oligosaccharide units, the core oligosaccharide and the lipid A, which harbors the endotoxic activity of the entire molecule. R-form LPS synthesized by the so-called rough (R) mutants of Gram-negative bacteria lacks the O-specific chain. Furthermore, the core-oligosaccharide may be present in different degrees of completion, depending on the class (Ra to Re) to which the mutant belongs. LPS are amphipathic molecules whose hydrophobicity decreases with increasing length of the sugar part. Based upon these differences, S- and R-form LPS show marked differences in the kinetics of their blood clearance and cellular uptake as well as in the ability to induce oxidative burst in human granulocytes and to activate the host complement system.
Safety information: 
MSDS: MSDS
Hazard information: Non-hazardous, Warning
Additional information: 
Synonyms: IAX-100-006-C500; Innaxon
Attachment to erythrocytes of uniform salt forms of lipopolysaccharides from Salmonella abortus-equi and its inhibition by various animal sera: M.D. Praino, et al.; Immunol. Commun. 8, 85 (1979) Read more
Preparation and properties of a standardized lipopolysaccharide from salmonella abortus equi: C. Galanos, et al.; Zentralbl. Bakteriol. Orig. A. 243, 226 (1979) Read more
Large-scale fractionation of S-form lipopolysaccharide from Salmonella abortus equi. Chemical and serological characterization of the fractions: C. Galanos, et al.; J. Chromatogr. 440, 397 (1988) Read more
Immunoblot analysis of the R-form lipopolysaccharide from Salmonella S forms: S. Schlecht, et al.; Zentralbl. Bakteriol. 277, 288 (1992) Read more
Differential clearance and induction of host responses by various administered or released lipopolysaccharides: R. Hasunuma, et al.; J. Endotoxin Res. 7, 421 (2001) Read more