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% Signal to Background of Continuous Real-Time TR-FRET MDM2 titration (autoubiquitination): Serial dilutions of GST-MDM2 from 200nM to 12.5nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-ATP.
% Signal to Background of Continuous Real-Time TR-FRET MDM2 titration (autoubiquitination): Serial dilutions of GST-MDM2 from 200nM to 12.5nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-ATP.
% Signal to Background of Continuous Real-Time TR-FRET MDM2 titration (autoubiquitination): Serial dilutions of GST-MDM2 from 200nM to 12.5nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-ATP.

MDM2 E3 Ligase TR-FRET Kit

Research Use Only
SBB-KF0030
South Bay Bio
Product group Chemicals
Price on request
Packing Size
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Overview

  • Supplier
    South Bay Bio
  • Product Name
    MDM2 E3 Ligase TR-FRET Kit
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Hazard Information
    Non-hazardous
  • Scientific Description
    South Bay Bios MDM2 TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto GST-MDM2 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto MDM2 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate true signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts. - South Bay Bios MDM2 TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto GST-MDM2 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto MDM2 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate true signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts. The kit uses ubiquitin labeled with either Europium-Cryptate or Cy5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto MDM2 leads to an increase in fluorescence emission at 665nm (Em-Acceptor) and decrease at emission wavelength 620nm (Em-Donor).
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352200