MDM2 E3 Ligase TR-FRET Kit

Catalog number: SBB-KF0030-KI01
Brand: South Bay Bio
Packing: 1 kit
Price: € 964.00
Expected delivery time: 7 days
% Signal to Background of Continuou...

Product specifications for - MDM2 E3 Ligase TR-FRET Kit

Product group: Chemicals
Category: Other
Species: Human
Datasheet: Datasheet
  Research Use Only
UNSPSC: 12352200
Storage instructions: -20-¦C, -80-¦C
Scientific information: 
Scientific info: South Bay Bios MDM2 TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto GST-MDM2 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto MDM2 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate true signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts. The kit uses ubiquitin labeled with either Europium-Cryptate or Cy5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto MDM2 leads to an increase in fluorescence emission at 665nm (Em-Acceptor) and decrease at emission wavelength 620nm (Em-Donor).
Safety information: 
Hazard information: Non-hazardous
Additional information: 
Synonyms: SBB-KF0030-KI01; South Bay Bio
Real-Time Homogenous TR-FRET Ubiquitin Conjugation and Deconjugation Assay Platform: I. Tomasic: Poster, EMBO Conference Ubiquitin and SUMO, Cavtat-Dubrovnik (2017) Read more