Chicken Anti-Rat IgG Secondary Antibody (AP)

LifeSpan BioSciences

Chicken Anti-Rat IgG Secondary Antibody (AP)

23

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Chicken Serum, Rat IgG and Rat Serum.

Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) catalog no. NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1400 to 1:6100 of the stated concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ELISA (1:2000 - 1:10000), IHC (1:200 - 1:1000), WB (1:500 - 1:2500) Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) catalog no. NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1400 to 1:6100 of the stated concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Polyclonal

1.2 mg/ml

Alk. Phosphatase

...

Chicken

IgG

Rat

2°C to 8°C

12352203