Chk1 antibody [DCS-310]

GeneTex

Chk1 antibody [DCS-310]

9

IHC-P: Use at a concentration of 1 microg/ml. Heat treatment is neccessary for paraffin embedded sections. Heat in a microwave oven, twice for 10 minutes in citrate buffer, pH 6.5.IP: Use at a concentration of 2 microg per 0.2 ml of cell extract. WB: Use at a concentration of 1 microg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). ECL detection is used.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

DCS-310

0.2 mg/ml

Unconjugated

CHEK1

checkpoint kinase 1

cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; CHK1; CHK1 checkpoint homolog; Chk1-S; serine/threonine-protein kinase Chk1

Mouse

IgG3

Serine/threonine-protein kinase Chk1

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Human, Mouse, Rat

-20°C or -80°C,2°C to 8°C

12352203