ICC/IF analysis of COS7 cells transiently transfected with Chk2 plasmid using GTX84691 Chk2 antibody [5C4].
![ICC/IF analysis of HT29 cells using GTX84691 Chk2 antibody [5C4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84691/GTX84691_1191_ICCIF_w_23061420_209.webp "ICC/IF analysis of HT29 cells using GTX84691 Chk2 antibody [5C4].")
![IHC-P analysis of human breast tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84691/GTX84691_3022_IHC-P_w_23061420_256.webp "IHC-P analysis of human breast tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human colon tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84691/GTX84691_3023_IHC-P_w_23061420_169.webp "IHC-P analysis of human colon tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human kidney tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84691/GTX84691_3024_IHC-P_w_23061420_337.webp "IHC-P analysis of human kidney tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human liver tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84691/GTX84691_3025_IHC-P_w_23061420_754.webp "IHC-P analysis of human liver tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human lung tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84691/GTX84691_3026_IHC-P_w_23061420_823.webp "IHC-P analysis of human lung tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human pancreas tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84691/GTX84691_3027_IHC-P_w_23061420_542.webp "IHC-P analysis of human pancreas tissue using GTX84691 Chk2 antibody [5C4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IP analysis of DDDDK tagged Chk2 overexpressed HEK293T lysate using GTX84691 Chk2 antibody [5C4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84691/GTX84691_3340_IP_w_23061420_455.webp "IP analysis of DDDDK tagged Chk2 overexpressed HEK293T lysate using GTX84691 Chk2 antibody [5C4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of wild type and Chk2 knock out HeLa cell lysate(10microg per lane) using GTX84691 Chk2 antibody [5C4]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84691/GTX84691_4558_20181228_WB_w_23061420_311.webp "WB analysis of wild type and Chk2 knock out HeLa cell lysate(10microg per lane) using GTX84691 Chk2 antibody [5C4]. Dilution : 1:500")
ICC/IF analysis of COS7 cells transiently transfected with Chk2 plasmid using GTX84691 Chk2 antibody [5C4].
Chk2 antibody [5C4]
GTX84691
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human
TargetCHEK2
Overview
- SupplierGeneTex
- Product NameChk2 antibody [5C4] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:1000. ICC/IF: 1:50-1:100. IHC-P: 1:150. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5C4
- Concentration1.43 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID11200
- Target nameCHEK2
- Target descriptioncheckpoint kinase 2
- Target synonymsCDS1; cds1 homolog; checkpoint-like protein CHK2; CHK2; CHK2 checkpoint homolog; hCds1; HuCds1; LFS2; PP1425; RAD53; serine/threonine-protein kinase Chk2
- HostMouse
- IsotypeIgG1
- Protein IDO96017
- Protein NameSerine/threonine-protein kinase Chk2
- Scientific DescriptionRegulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on Ser-216, preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at Thr-18 and Ser-20.
- ReactivityCanine, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203