Chromogranin A Antibody Cocktail

Biorbyt

Chromogranin A Antibody Cocktail

16

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT IHC-P

Research Use Only

Monoclonal

LK2H10 + PHE5 + CGA414

Unconjugated

CHGA

chromogranin A

betagranin (N-terminal fragment of chromogranin A); catestatin; CGA; chromofungin; chromogranin-A; parathyroid secretory protein 1; pituitary secretory protein I; SP-I

Mouse

IgG1

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Human, Mouse, Rat

-20°C,2°C to 8°C

12352203