Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CK7 Polyclonal Antibody(bs-1610R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CK7 Polyclonal Antibody(bs-1610R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
CK7 Polyclonal Antibody
BS-1610R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKRT7
Overview
- SupplierBioss Antibodies
- Product NameCK7 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID3855
- Target nameKRT7
- Target descriptionkeratin 7
- Target synonymsCK7; CK-7; cytokeratin 7; K2C7; K7; keratin 7, type II; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; keratin, type II cytoskeletal 7; sarcolectin; SCL; type II mesothelial keratin K7; type-II keratin Kb7
- HostRabbit
- IsotypeIgG
- Protein IDP08729
- Protein NameKeratin, type II cytoskeletal 7
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Expression of CD133 in endometrial cancer cells and its implications. Ding DC et al., 2017, J CancerRead more
- Extranuclear Translocation of High-Mobility Group A1 Reduces the Invasion of Extravillous Trophoblasts Involved in the Pathogenesis of Preeclampsia: New Aspect of High-Mobility Group A1. Uchikura Y et al., 2017 Dec, Reprod SciRead more
- Phosphoinositide 3-Kinase (PI3K) Subunit p110delta Is Essential for Trophoblast Cell Differentiation and Placental Development in Mouse. Hu X et al., 2016 Jun 16, Sci RepRead more
- Immune activation during the implantation phase causes preeclampsia-like symptoms via the CD40-CD40 ligand pathway in pregnant mice. Matsubara K et al., 2016 Jun, Hypertens ResRead more
- Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion. Chen H et al., 2015, PLoS OneRead more
- IFN-gamma inhibits osteopontin expression in human decidual stromal cells and can be attenuated by 1alpha,25-dihydroxyvitamin D3. Hu B et al., 2012 Oct, Am J Reprod ImmunolRead more