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ProductsBack/CKB / Creatine Kinase BB Antibody (aa62-111, Biotin)

CKB / Creatine Kinase BB Antibody (aa62-111, Biotin)

LS-C471364
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCKB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CKB / Creatine Kinase BB Antibody (aa62-111, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID1152
  • Target name
    CKB
  • Target description
    creatine kinase B
  • Target synonyms
    BCK; B-CK; brain creatine kinase; CKBB; CPK-B; creatine kinase B chain; creatine kinase brain; creatine kinase brain-type; creatine kinase B-type; creatine phosphokinase B-type; epididymis luminal protein 211; epididymis secretory protein Li 29; HEL-211; HEL-S-29
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CKB using anti-CKB antibody (A01695-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: human MDA-MB-231 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CKB antigen affinity purified polyclonal antibody (Catalog # A01695-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CKB at approximately 45KD. The expected band size for CKB is at 43KD.
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