IHC-P analysis of human breast carcinoma tissue using GTX22731 Clathrin heavy chain antibody [X22]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
![IHC-P analysis of human colon tissue using GTX22731 Clathrin heavy chain antibody [X22]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22731/GTX22731_1086_IHC-P_w_23060620_698.webp "IHC-P analysis of human colon tissue using GTX22731 Clathrin heavy chain antibody [X22]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
![WB analysis of 25 ug of human brain lysates using GTX22731 Clathrin heavy chain antibody [X22]. Dilution : 1:300](https://www.genetex.com/upload/website/prouct_img/normal/GTX22731/GTX22731_1577_WB_w_23060620_365.webp "WB analysis of 25 ug of human brain lysates using GTX22731 Clathrin heavy chain antibody [X22]. Dilution : 1:300")
![ICC/IF analysis of HeLa cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22731/GTX22731_398_ICC-IF_w_23060620_239.webp "ICC/IF analysis of HeLa cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of NCI-H460 Cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22731/GTX22731_399_ICC-IF_w_23060620_800.webp "ICC/IF analysis of NCI-H460 Cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of U251 cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22731/GTX22731_400_ICC-IF_w_23060620_636.webp "ICC/IF analysis of U251 cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human breast carcinoma tissue using GTX22731 Clathrin heavy chain antibody [X22]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
Clathrin heavy chain antibody [X22]
GTX22731
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application
Product group Antibodies
ReactivityBovine, Canine, Hamster, Human, Mouse, Primate, Rat
TargetCLTC
Overview
- SupplierGeneTex
- Product NameClathrin heavy chain antibody [X22] - KO/KD Validated
- Delivery Days Customer9
- Antibody SpecificityElectron microscopy and proteolysis mapping demonstrate that MA1-065 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
- Application Supplier NoteWB: 1:100-1:500. ICC/IF: 1:1,000. IHC-P: 1:100. FACS: 1/200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDX22
- Concentration6 mg/ml
- ConjugateUnconjugated
- Gene ID1213
- Target nameCLTC
- Target descriptionclathrin heavy chain
- Target synonymsCHC; CHC17; clathrin heavy chain 1; clathrin heavy chain on chromosome 17; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH-17; CLTCL2; Hc; MRD56
- HostMouse
- IsotypeIgG1
- Protein IDQ00610
- Protein NameClathrin heavy chain 1
- Scientific DescriptionClathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
- ReactivityBovine, Canine, Hamster, Human, Mouse, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Convallatoxin enhance the ligand-induced mu-opioid receptor endocytosis and attenuate morphine antinociceptive tolerance in mice. Chao PK et al., 2019 Feb 20, Sci RepRead more
- Protease-activated Receptor-4 Signaling and Trafficking Is Regulated by the Clathrin Adaptor Protein Complex-2 Independent of beta-Arrestins. Smith TH et al., 2016 Aug 26, J Biol ChemRead more
- Cell surface epidermal growth factor receptors increase Src and c-Cbl activity and receptor ubiquitylation. Parks EE et al., 2014 Sep 12, J Biol ChemRead more
- Size-dependent toxicity and cell interaction mechanisms of gold nanoparticles on mouse fibroblasts. Coradeghini R et al., 2013 Mar 13, Toxicol LettRead more
- Phosphorylation of protease-activated receptor-2 differentially regulates desensitization and internalization. Ricks TK et al., 2009 Dec 4, J Biol ChemRead more