CLP1 antibody [N3C3]

GeneTex

CLP1 antibody [N3C3]

9

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.27 mg/ml

Unconjugated

CLP1

cleavage factor polyribonucleotide kinase subunit 1

ATP/GTP-binding protein; cleavage and polyadenylation factor I subunit 1; hClp1; HEAB; homolog of yeast CFIA subunit Clp1p; polyadenylation factor Clp1; polynucleotide kinase Clp1; polyribonucleotide 5'-hydroxyl-kinase Clp1; pre-mRNA cleavage complex II protein Clp1

Rabbit

IgG

Polyribonucleotide 5'-hydroxyl-kinase Clp1

Polynucleotide kinase that can phosphorylate the 5-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5-terminus of the tRNA 3-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3-end formation. Also phosphorylates the 5-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Human

-20°C or -80°C,2°C to 8°C

12352203