Collagen Substrate, FITC Conjugate
X1098
ApplicationsFunctional Assay
Product group Proteins / Signaling Molecules
Overview
- SupplierNordic-MUbio
- Product NameCollagen Substrate, FITC Conjugate
- Delivery Days Customer7
- Application Supplier NoteUsed for the assay of mammalian collagenase activity. To heat denature collagen for preparing a substrate for gelatinase, transfer collagen solution to a brown glass bottle and heat to 80C. Collagen-fluorescein may be used as a substrate for the determination of collagenase activity by quantitative fluorescent assay of lysed collagen. Excitation is 490 nm, emission is 520 nm. 1) Dialyze at 5°C vs. 0.1 M Tris pH 7.5 containing 0.2 M NaCl. 2.) Follow procedure of Baiciet. al. Anal. Biochem., vol 108, pg. 230 (1980). The collagen-FITC is solubilized with collagenase at pH 8.0 at 37°C and absorbance at 494 nm determined in a 10 mm cell. Using = 7200 at pH 8 at 494 nm; umoles FITC per mg = 0.24.
- ApplicationsFunctional Assay
- Applications SupplierSubstrate
- Category SupplierProteins and peptides
- CertificationResearch Use Only
- Concentration1 mg/ml
- ConjugateFITC
- Estimated Purity>95%
- Scientific DescriptionExtracted from bovine skin
- Shelf life instructionStable in acidic buffer when stored at -20ºC, however slowly degrades when stored in under neutral conditions.
- SourceBovine skin We certify that: a) The collagen fluorescein is of animal origin only. b) The collagen fluorescein was derived only from animals which were slaughtered in the United States in USDAinspected abattoirs where the animals were subjected to ...
- UNSPSC12352202
References
- 1. Terato, K., et al. 'A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrate.' Biochim. Biophys. Acta 1976, 445, 753-762 2. Sellers, A., et al. 'Evidence that latent collagenases are enzyme-inhibitor complexes.' Biochem. J. 1977, 163, 303-307 3. Shinkai, H., et al. 'A complex of collagenase with low molecular weight inhibitors in the culture medium of embryonic chick skin explants.' J. Biochem. (Tokyo) 1977, 81, 261-263 4. Shinkai, H. & Nagai, Y., 'A latent collagenase from embryonic human skin explants.' J. Biochem. (Tokyo) 1977, 81, 1261-1263 5. Murawaki, Y., et al. 'Serum collagenase activity in patients with chronic liver disease.' J. Hepatol. 1993, 328-334 6. Baici, A., Cohen, G., Fehr, K. and Boni, A., Anal. Biochem., 108, 230, (1980).