Conjugation-Ready HRP Maleimide

Biorbyt

Conjugation-Ready HRP Maleimide

16

1. Coat antibody or antigen onto the ELISA plate using ICTs Antibody Coating Buffer or Antigen Coating Buffer., 2. Incubate 8-24 hours at room temperature (RT)., 3. Aspirate the coating solution., 4. Wash each well twice with ICTs ELISA Wash Buffer., 5. Block the uncoated regions of the ELISA plate by pipetting 300-400 microL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution., 6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT., 7. Aspirate the blocking buffer; do not wash., 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Research Use Only

Uses maleimide-activated HRP to create stable protein-HRP conjugates. Allows control of the HRP to protein ratio. Avoids HRP inactivation seen with periodate oxidation methods and protein aggregation / precipitation seen with glutaraldehyde methods.

2°C to 8°C

41116133