Cortactin (phospho Tyr421) antibody

GeneTex

Cortactin (phospho Tyr421) antibody - Orthogonal Validated

9

WB: 1:250. ICC/IF: 1:250. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry

Research Use Only

Polyclonal

Unconjugated

Cttn

cortactin

1110020L01Rik; Ems; Ems1; mammary tumor and squamous cell carcinoma associated (p80/85 src substrate); src substrate cortactin

Rabbit

IgG

Src substrate cortactin

Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks.

Human, Mouse

-20°C or -80°C,2°C to 8°C

12352203