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COX5B Antibody (YA2550)

HY-P82805
MedChem Express
TargetCOX5B
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    COX5B Antibody (YA2550)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1329
  • Target name
    COX5B
  • Target description
    cytochrome c oxidase subunit 5B
  • Target synonyms
    COXVB; cytochrome c oxidase polypeptide VB, mitochondrial; cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase subunit Vb; epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    COX5B Antibody (YA2550) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2550), targeting COX5B, with a predicted molecular weight of 14 kDa (observed band size: 14 kDa). COX5B Antibody (YA2550) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human, mouse, rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COX5B using anti-COX5B antibody (A06090-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human MCF-7 whole cell lysates. Lane 7: human HCCT tissue lysates, Lane 8: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX5B antigen affinity purified polyclonal antibody (Catalog # A06090-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX5B at approximately 15 kDa. The expected band size for COX5B is at 15 kDa.
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