CSF1 / MCSF Antibody

LifeSpan BioSciences

CSF1 / MCSF Antibody

23

Human M-CSF

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hM-CSF (7 ng/ml), a concentration of 0.02-0.03 ug/ml of this antibody is required. ELISA: To detect hM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human M-CSF (LS-C104655) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hM-CSF (7 ng/ml), a concentration of 0.02-0.03 ug/ml of this antibody is required. ELISA: To detect hM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human M-CSF (LS-C104655) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA

Research Use Only

Polyclonal

Unconjugated

...

CSF1

colony stimulating factor 1

colony stimulating factor 1 (macrophage); CSF-1; lanimostim; macrophage colony stimulating factor 1; macrophage colony-stimulating factor 1; MCSF

Rabbit

Human

-20°C,2°C to 8°C,RT

12352203